- Investigate coral symbiont communities from the world’s warmest reefs.
- This study will use an integrative approach, combining molecular, genomic, and physiological methodologies. You will have the opportunity to learn new approaches and apply cutting-edge technologies in your research.
- You will gain mechanistic insights into symbiont thermal tolerance that can be used to improve human interventions into the coral reef crisis.
Mass coral bleaching events associated with thermal stress have caused substantial declines in coral ecosystems. Coral bleaching results from the breakdown in the relationship between the coral and its symbiotic algae of the family Symbiodiniaceae and can occur at temperatures just 1-2°C above the average summer maxima (Goreau et al., 2000). Nevertheless, it has been shown that changing the dominant association to a thermally tolerant symbiont can increase the coral holobiont’s bleaching threshold by up to 1.5°C (Berkelmans & Van Oppen, 2006). As such, there is substantial interest in identifying novel thermally tolerant symbionts and understanding the mechanisms that enhance thermal tolerance.
This project will focus on Symbiodiniaceae communities associated with the coral reefs of the Persian/Arabian Gulf (PAG). This young sea is home to the world’s warmest coral reefs and the coral-algal associations have the highest known bleaching thresholds (Riegl et al., 2011). Interestingly, the reefs of the southern PAG are largely devoid of the most widely reported stress tolerant symbiont species Durusdinium trenchii (Hume et al., 2015). While widespread associations with Cladocopium thermophilum and Symbiodinium spp. symbionts have been documented in the PAG (Smith et al., 2017a; Smith et al., 2017b; Smith et al., 2017c; Howells et al., 2020), little is known about the underlying mechanisms that facilitate symbiont thermal tolerance in this region. In this project, you will combine next generation sequencing with photophysiological approaches to uncover a mechanistic understanding of symbiont thermal tolerance that can be used to help further efforts to maintain reefs in the face of climate change.
Figure 1: Mortality associated with coral bleaching. Within four months, this coral colony died as a result of a bleaching event where water temperatures exceeded 37°C. In this project, your research will help identify how some coral symbionts are able to maintain a successful symbiosis under high temperatures. Image from Smith et al. 2017c.
HostUniversity of Warwick
- Organisms and Ecosystems
In this project, you will likely combine fieldwork, laboratory experiments, and computational approaches. Long-read sequencing, genome assembly, and comparative genomics will be used to investigate coral symbiont adaptation to extreme reef temperatures. Alongside the genomics approaches, culturing, thermal stress experiments, and chlorophyll fluorescence measurements will be used to compare physiological responses between thermally tolerant and sensitive symbionts.
Training and skills
Students will be awarded CENTA2 Training Credits (CTCs) for participation in CENTA2-provided and ‘free choice’ external training. One CTC equates to 1⁄2 day session and students must accrue 100 CTCs across the three years of their PhD.
Training will be provided in a range of different techniques, as required, including molecular biology (e.g., high molecular weight DNA extractions, PCR, sequencing library preparation), bioinformatics (e.g., genome assembly, comparative genomics), and experimental biology (e.g., experimental design, algal culturing, chlorophyll fluorometry).
Partners and collaboration
This project will involve collaboration with Dr. John Burt and members of Burt Lab at New York University Abu Dhabi.
Further details on how to contact the supervisor for this project and how to apply for this project can be found here:
Please see the lab page here.
To learn more about the project, contact Ed Smith ([email protected]). Please include a CV, details of past research, and outline your interest in the project.
To apply to this project:
- You must include a CENTA studentship application form, downloadable from: CENTA Studentship Application Form 2024.
- You must include a CV with the names of at least two referees (preferably three) who can comment on your academic abilities.
- Please submit your application and complete the host institution application process via: https://warwick.ac.uk/fac/sci/lifesci/study/pgr/studentships/nerccenta/ Complete the online application form – selecting course code P-C1PB (Life Sciences PhD); from here you will be taken through to another screen where you can select your desired project. Please enter “NERC studentship” in the Finance section and add Nikki Glover, [email protected] as the scholarship contact. Please also complete the CENTA application form 2024 and submit via email to [email protected]. Please quote CENTA 2024-W4 when completing the application form.
Applications must be submitted by 23:59 GMT on Wednesday 10th January 2024.
Survey the symbiont communities in the southern PAG and establish algal cultures.
Compare the thermal physiology of your cultured strains to strains collected from other regions.
Assemble the genome of a thermally tolerant symbiont from the PAG and compare to other Symbiodiniaceae genomes.
Aranda, M., Li, Y., Liew, Y.J., Baumgarten, S., Simakov, O., Wilson, M.C., Piel, J., Ashoor, H., Bougouffa, S., Bajic, V.B. and Ryu, T. (2016) Genomes of coral dinoflagellate symbionts highlight evolutionary adaptations conducive to a symbiotic lifestyle. Scientific reports, 6(1), p.39734.
Berkelmans, R. and Van Oppen, M.J. (2006) The role of zooxanthellae in the thermal tolerance of corals: a ‘nugget of hope’ for coral reefs in an era of climate change. Proceedings of the Royal Society B: Biological Sciences, 273(1599), pp.2305-2312.
González-Pech, R.A., Stephens, T.G., Chen, Y., Mohamed, A.R., Cheng, Y., Shah, S., Dougan, K.E., Fortuin, M.D., Lagorce, R., Burt, D.W. and Bhattacharya, D. (2021) Comparison of 15 dinoflagellate genomes reveals extensive sequence and structural divergence in family Symbiodiniaceae and genus Symbiodinium. BMC biology, 19, pp.1-22.
Goreau, T., McClanahan, T., Hayes, R. and Strong, A.L. (2000) Conservation of coral reefs after the 1998 global bleaching event. Conservation biology, 14(1), pp.5-15.
Howells, E.J., Bauman, A.G., Vaughan, G.O., Hume, B.C., Voolstra, C.R. and Burt, J.A. (2020) Corals in the hottest reefs in the world exhibit symbiont fidelity not flexibility. Molecular Ecology, 29(5), pp.899-911.
Hume, B.C., D’Angelo, C., Smith, E.G., Stevens, J.R., Burt, J. and Wiedenmann, J. (2015) Symbiodinium thermophilum sp. nov., a thermotolerant symbiotic alga prevalent in corals of the world’s hottest sea, the Persian/Arabian Gulf. Scientific reports, 5(1), p.8562.
Liu, H., Stephens, T.G., González-Pech, R.A., Beltran, V.H., Lapeyre, B., Bongaerts, P., Cooke, I., Aranda, M., Bourne, D.G., Forêt, S. and Miller, D.J. (2018) Symbiodinium genomes reveal adaptive evolution of functions related to coral-dinoflagellate symbiosis. Communications biology, 1(1), p.95.
Riegl, B.M., Purkis, S.J., Al-Cibahy, A.S., Abdel-Moati, M.A. and Hoegh-Guldberg, O. (2011) Present limits to heat-adaptability in corals and population-level responses to climate extremes. PloS one, 6(9), p.e24802.
Smith, E.G., Hume, B.C., Delaney, P., Wiedenmann, J. and Burt, J.A. (2017) Genetic structure of coral-Symbiodinium symbioses on the world’s warmest reefs. PloS one, 12(6), p.e0180169.
Smith, E.G., Ketchum, R.N. and Burt, J.A. (2017) Host specificity of Symbiodinium variants revealed by an ITS2 metahaplotype approach. The ISME Journal, 11(6), pp.1500-1503.
Smith, E.G., Vaughan, G.O., Ketchum, R.N., McParland, D. and Burt, J.A. (2017) Symbiont community stability through severe coral bleaching in a thermally extreme lagoon. Scientific Reports, 7(1), p.2428.